For the 2021 season, Division III realigned football into six regions. Region 3 is primarily teams South and West of Harrisburg, Pennsylvania. To find teams from the Centennial Conference and Presidents' Athletic Conference, look in Region 2.
| School |
Nickname |
Location |
Conference |
Region |
Surface |
Enrollment |
| Austin |
Kangaroos |
Sherman, TX |
ASC |
3 |
Turf |
1204 |
| Averett |
Cougars |
Danville, VA |
ODAC |
3 |
Turf |
977 |
| Belhaven |
Blazers |
Jackson, MS |
USAC |
3 |
Turf |
1104 |
| Berry |
Vikings |
Mount Berry, GA |
SAA |
3 |
Turf |
2157 |
| Birmingham-Southern |
Panthers |
Birmingham, AL |
SAA |
3 |
Turf |
1039 |
| Brevard |
Tornados |
Brevard, NC |
USAC |
3 |
Turf |
713 |
| Bridgewater |
Eagles |
Bridgewater, VA |
ODAC |
3 |
Turf |
1868 |
| Centre |
Colonels |
Danville, KY |
SAA |
3 |
Turf |
1337 |
| East Texas Baptist |
Tigers |
Marshall, TX |
ASC |
3 |
Turf |
1230 |
| Ferrum |
Panthers |
Ferrum, VA |
ODAC |
3 |
Turf |
1030 |
| Greensboro |
Pride |
Greensboro, NC |
USAC |
3 |
Turf |
696 |
| Guilford |
Quakers |
Greensboro, NC |
ODAC |
3 |
Turf |
1266 |
| Hampden-Sydney |
Tigers |
Hampden-Sydney, VA |
ODAC |
3 |
Grass |
986 |
| Hardin-Simmons |
Cowboys |
Abilene, TX |
ASC |
3 |
Turf |
1411 |
| Hendrix |
Warriors |
Conway, AR |
SAA |
3 |
Turf |
1101 |
| Howard Payne |
Yellow Jackets |
Brownwood, TX |
ASC |
3 |
Turf |
738 |
| Huntingdon |
Hawks |
Montgomery, AL |
USAC |
3 |
Turf |
830 |
| LaGrange |
Panthers |
LaGrange, GA |
USAC |
3 |
Turf |
844 |
| Lyon |
Scots |
Batesville, AR |
IND |
3 |
|
572 |
| Mary Hardin-Baylor |
Crusaders |
Belton, TX |
ASC |
3 |
Turf |
3112 |
| Maryville (Tenn.) |
Scots |
Maryville, TN |
USAC |
3 |
Grass |
1061 |
| McMurry |
War Hawks |
Abilene, TX |
ASC |
3 |
Turf |
1019 |
| Methodist |
Monarchs |
Fayetteville, NC |
USAC |
3 |
Grass |
1974 |
| Millsaps |
Majors |
Jackson, MS |
SAA |
3 |
Turf |
768 |
| N.C. Wesleyan |
Battling Bishops |
Rocky Mount, NC |
USAC |
3 |
Grass |
1600 |
| Randolph-Macon |
Yellow Jackets |
Ashland, VA |
ODAC |
3 |
Turf |
1452 |
| Rhodes |
Lynx |
Memphis, TN |
SAA |
3 |
Turf |
1939 |
| Sewanee |
Tigers |
Sewanee, TN |
SAA |
3 |
Turf |
1669 |
| Shenandoah |
Hornets |
Winchester, VA |
ODAC |
3 |
Turf |
1989 |
| Southern Virginia |
Knights |
Buena Vista, VA |
USAC |
3 |
Turf |
1016 |
| Southwestern |
Pirates |
Georgetown, TX |
ASC |
3 |
Turf |
1495 |
| Sul Ross State |
Lobos |
Alpine, TX |
ASC |
3 |
Turf |
1179 |
| Texas Lutheran |
Bulldogs |
Seguin, TX |
ASC |
3 |
Turf |
1379 |
| Trinity (Texas) |
Tigers |
San Antonio, TX |
SAA |
3 |
Turf |
2457 |
| Washington and Lee |
Generals |
Lexington, VA |
ODAC |
3 |
Turf |
1843 |