Through games of Oct. 1, 2016:
| # | School (No. 1 votes) | Rec | Pts | Prev. |
| 1 | Mount Union (16) | 4-0 | 608 | 1 |
| 2 | UW-Whitewater (1) | 4-0 | 589 | 2 |
| 3 | Mary Hardin-Baylor (7) | 4-0 | 584 | 3 |
| 4 | St. Thomas (1) | 5-0 | 565 | 4 |
| 5 | UW-Oshkosh | 4-0 | 524 | 5 |
| 6 | North Central (Ill.) | 4-0 | 471 | 6 |
| 7 | UW-Platteville | 3-1 | 429 | 8 |
| 8 | Linfield | 2-1 | 424 | 7 |
| 9 | Johns Hopkins | 5-0 | 404 | 9 |
| 10 | Wheaton (Ill.) | 5-0 | 398 | 11 |
| 11 | St. John's | 4-1 | 397 | 10 |
| 12 | Hardin-Simmons | 4-0 | 393 | 12 |
| 13 | Salisbury | 4-0 | 320 | 13 |
| 14 | St. John Fisher | 5-0 | 241 | 14 |
| 15 | Thomas More | 4-1 | 228 | 16 |
| 16 | Wittenberg | 4-0 | 222 | 18 |
| 17 | East Texas Baptist | 4-0 | 214 | 17 |
| 18 | Franklin | 4-1 | 173 | 19 |
| 19 | Stevenson | 5-0 | 156 | 20 |
| 20 | Wabash | 3-1 | 113 | 23 |
| 21 | St. Lawrence | 5-0 | 106 | 25 |
| 22 | Hobart | 5-0 | 94 | 21 |
| 23 | Dubuque | 5-0 | 64 | -- |
| 24 | Hendrix | 4-0 | 51 | -- |
| 25 | Alfred | 5-0 | 49 | -- |
Dropped out: No. 15 Huntingdon; No. 22 Christopher Newport; No. 24 Central.
Others receiving votes: Delaware Valley 45; DePauw 40; John Carroll 40; Claremont-Mudd-Scripps 33; Guilford 32; Whitworth 21; Case Western Reserve 20; UW-Stevens Point 16; Monmouth 15; Huntingdon 11; Amherst 7; Christopher Newport 7; Wesley 6; Coe 5; Central 3; Washington and Jefferson 3; Berry 2; Western New England 1; Westminster (Pa.) 1.
The D3football.com Top 25 is voted on by a panel of 25 coaches, Sports Information Directors and media members from across the country, and is published weekly.